Real Time PCR NRTP-500 supports a 96-well sample capacity for efficient high-throughput testing. It accommodates reaction volumes ranging from 10 to 50μl for flexible assay setup. This unit uses an electronic, automatic heated lid to ensure consistent and secure sample sealing. It achieves fast heating rates of up to 6°C/s for rapid thermal cycling. Our PCR system enables multiplex detection of five fluorescent dyes in a single well for detailed data collection.
$18,200.00
Specifications
| Sample capacity | 96 wells |
| Reaction volume | 10 to 50μl |
| Heating/cooling method | Peltier |
| Maximum ramp rate | 6℃/s |
| Temperature setting range | 4 to 100℃ |
| Temperature accuracy | ±0.2℃ |
| Temperature uniformity | ±0.2℃ |
| Gradient zone | 12 columns |
| Gradient range | 1 to 36℃ |
| Heated lid | Electronic automatic lid |
| Excitation source | Long-life, high-performance LEDs |
| Detector | Highly sensitive MPPC with a Fresnel lens |
| Scanning principle | Time-resolved scanning technology |
| Detector position | Top of the block |
| Excitation/detection range | 455 to 650nm/510 to 715nm |
| Fluorescence channel | 4 |
| Detection sensitivity | 1 copy of the target sequence |
| System sensitivity | Detect differences as small as 1.33-fold in target quantities in singleplex reactions |
| Dynamic range | 10 orders of magnitude |
| Dye compatibility | FAM/SYBR Green, VIC/JOE/HEX/TET, NED/TAMRA/Cy3 JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ |
| Data analysis modes | Absolute quantification, Relative quantification, Endpoint qualitative analysis, Melt curve analysis, Protein Stability Screening, Genotyping |
Features
Applications
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FAQ for Real Time PCR NRTP-500
1: Is the Real Time PCR NRTP-500 compatible with different sample formats?
Yes, our Real Time PCR system supports low-profile 96-well plates, 8-strip tubes, and individual tubes. This flexibility allows use with various sample types and workflow preferences.
2: What kind of optical system does the Real Time PCR NRTP-500 use?
Our Real Time PCR system uses a patented detection system combining a Fresnel lens and a high-sensitivity MPPC. This setup minimizes cross-talk and enhances fluorescence signal clarity.
3: How does the Real Time PCR system ensure consistent sample sealing?
The Real Time PCR system uses an electronic, automatic heated lid that applies uniform pressure across all wells. This prevents evaporation and ensures consistent reaction conditions throughout the run.
4: Does the Real Time PCR NRTP-500 require regular maintenance?
No, the Real Time PCR system is designed to be maintenance-free under normal operating conditions. It uses high-quality components like Peltier modules and a sealed optical path to ensure long-term stability and minimal upkeep.
5: What steps should be taken to keep the Real Time PCR system in good condition?
Keeping the Real Time PCR system in a clean, dust-free environment and ensuring proper ventilation will help extend its life. Regular software updates and gentle handling of the sample block and lid are also beneficial.
6: How accurate and reproducible are the results from this Real Time PCR system?
This system delivers high reproducibility with minimal variation between replicates. Its uniform thermal block design ensures consistent amplification across all wells, which is crucial for sensitive Real Time PCR analysis, including low-copy target detection.
7: How user-friendly is the software interface of this Real Time PCR system?
The software is designed for ease of use, featuring a navigational interface and project management tools. Users can quickly load saved protocols, import sample information, and initiate Real Time PCR runs with minimal input, reducing setup time and human error.
8: Is this Real Time PCR system suitable for point-of-care or field use?
Absolutely. Its lightweight, portable design and battery-powered operation make this Real Time PCR system perfect for point-of-care testing and mobile diagnostic units. It is engineered for stable performance even in remote or non-laboratory settings.
9: What steps should I take if the Real Time PCR system shows inconsistent results?
Ensure that sample volumes are uniform, reagents are fresh, and the instrument is placed on a stable surface. Verifying thermal cycling parameters and proper sealing of reaction tubes can also help improve Real Time PCR consistency.
Labnics Real Time PCR use fluorescent systems for accurate real-time DNA quantification. They support multiplex detection for simultaneous amplification of multiple targets. These units offer multi-melting curve analysis for reliable result confirmation. They come with built-in software for streamlined data analysis and reporting. Our Real-Time PCR includes data export capability for efficient result management and analysis.