Digital PCR System NDTP-100 features a single fluorescence channel compatible with both probe and dye detection methods. It supports a standard reaction volume of 15µl for flexible experimental setups. This unit uses a micro-cavity chip with solid-phase segmentation for accurate partitioning. It delivers rapid results with single-chip reading completed in 5 seconds. Our PCR system employs a high-efficiency LED light source for reliable fluorescence detection.
| Number of fluorescence channels |
1 |
| Sample detection throughput |
1/batch, can be read continuously without interruption |
| Daily detection throughput |
360 in a single day (8 hours) |
| Reaction volume |
15µl standard, adjustable within the 40µl range |
| Droplet preparation |
Pipetting without an additional microdroplet generation system |
| Sample preparation time |
≤10 seconds/piece |
| Sample detection time |
≤1 minute/piece |
| Detection sensitivity |
≤0.001%, can detect single-copy genes |
| Dynamic range |
≥5 orders of magnitude, 1 to 250000 copies/sample |
| Reagent versatility |
Compatible with the probe method and dye method |
| Compatible dyes |
FAM, SYBR Green, EvaGreen, VIC/HEX, and other dyes and similar wavelength dyes |
| Supporting reagents |
10× high-concentration DNA detection reagent and 5× RT-dPCR one-step RNA detection reagent |
| Maximum sample input |
≥12 µl |
| Excitation light source |
High-efficiency maintenance-free LED light source |
| Detector |
High-resolution CMOS sensor |
| Valid time of chip reading |
Read repeatedly within 2 weeks |
| Micro-reaction unit |
Micro-cavity chip, solid phase segmentation |
| Bio-chip type |
10k, 22k, 120k option, other types are customizable |
| Software |
Calculation of copy number, copy number concentration, mutation abundance, confidence interval range, accuracy; threshold line automatic or manual division, single or unified threshold division; output excel data, two-dimensional scatter plot, two-dimensional bar chart, three-dimensional space map; automatically identify complex droplet clusters, output chip actual hole position discrimination map; data quality control function, etc. automatically generate test reports |
| Data security |
Permission management, auditing, and electronic signature functions to ensure the validity and reliability of data and meet FDA 21 CFR Part 11 compliance requirements |
| Power supply |
220V/50Hz to 60Hz |
| Dimension |
Thermal cycler: 480×330×340mm
Biochip reader: 130×150×240mm |
| Net weight |
Thermal cycler: 8.9kg
Biochip reader: 1.7 kg |
FAQ for Digital PCR System NDTP-100
1: How fast can the Digital PCR System NDTP-100 deliver results?
The Digital PCR System is designed for speed, with sample preparation taking less than 10 seconds and single-chip reading completed in only 5 seconds. This efficiency helps maintain high throughput, with the ability to process up to 480 samples in a single day.
2: What pathogens and targets can it detect in clinical testing?
The Digital PCR System can accurately detect pathogens including HIV, HBV, HCV, nCoV, bacteria, fungi, mycoplasma, and chlamydia. It is also capable of detecting genetically modified organisms and performing food safety evaluations.
3: How long can chip data be stored and reused?
Chip data can be read and reanalyzed for up to two weeks without compromising accuracy. This feature provides flexibility for reviewing and validating results after the initial run.
4: How often does the Digital PCR System NDTP-100 require maintenance?
The Digital PCR System is designed for minimal maintenance, thanks to its high-efficiency, maintenance-free LED light source and robust construction. Routine care, such as keeping the biochip reader and thermal cycler clean, ensures consistent performance.
5: What steps should be taken to clean the Digital PCR System?
Cleaning should be performed using lint-free wipes and non-corrosive cleaning agents on external surfaces. The biochip holder and sample loading areas should be kept free of dust and residue to maintain optimal optical detection.
6: What types of samples can be tested with this Digital PCR System?
The Digital PCR System can handle a wide variety of samples, including clinical, environmental, and biopharmaceutical specimens. It is suitable for DNA, RNA, and other nucleic acid analyses. Its biochip technology ensures consistent results even with low-abundance targets.
7: How reliable are the results at low concentrations?
The Digital PCR System maintains high accuracy even at very low nucleic acid concentrations. Its advanced partitioning and biochip technology ensure reproducible results. Low-abundance targets are reliably quantified without errors.
8: How often should the Digital PCR System be cleaned?
The Digital PCR System should be cleaned regularly after each use to ensure optimal performance. Wiping the exterior and touchscreen with a soft, lint-free cloth and using recommended cleaning solutions for the biochip area prevents contamination and maintains accuracy.
9: What type of maintenance is required for the biochip technology?
Biochips should be handled carefully and stored as per the manufacturer’s instructions. Regular inspection for residue or damage and proper cleaning of the chip holder ensures consistent performance and prolongs its lifespan.
